Angiogenesis is the physiological process of formation and remodeling of new blood vessels and capillaries from pre-existing blood vessels. This can be achieved through endothelial sprouting or splitting of the vessels and capillaries.
MIMETAS scientists developed advanced and physiologically relevant models that included the growth and sprouting of vessels from a main perfused vessel into a collagen extracellular matrix as directed by pro- or anti-angiogenic factors. High-content imaging allows visualization of angiogenic structures, 3D reconstitution, and complex analysis of angiogenesis and sprouting of new blood vessels. Here, we describe the imaging and analysis methods for obtaining multiple quantitative descriptors of angiogenesis that could be used for comparative research into disease phenotypes and compound effects.
In this application note you will learn:
- How to visualize angiogenic sprouting and perform 3D reconstitution of structures
- How to perform quantitative assessment of angiogenesis, including number of sprouts, total volume, and average intensity
- How to generate physiologically-relevant results using the OrganoPlate® platform
Further reading:
- Can You Really Model Angiogenesis In Vitro?
- Dissatisfied With Your Angiogenesis Model? Read This!
- App Note: Perfused 3D angiogenic sprouting in a high-throughput microfluidic platform